Composition phytochimique et activité antibactérienne des extraits de Cajanus cajan (L.) Millsp., Vernonia amygdalina Delile et Psidium guayava L. utilisés dans le traitement des infections diarrhéiques au Bénin
AMADOU Afoussatou
Research Unit in applied microbiology and pharmacology of substances naturals
Poster
The present study was initiated in the general objective, to evaluate the chemical composition of the aqueous and ethanolic extracts of some plants of Southern Benin namely Cajanus cajan (leaves), Vernonia amygdalina (leaves) and Psidium guayava (leaves and roots) and their activities on multi-resistant bacterial strains.
The toxicity of the extracts was assessed by the larval toxicity test using Artemia salina larvae. The agar and liquid diffusion methods were used for the sensitivity test and the determination of the minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts on enteropathogenic strains.
Selected plants all had high levels of flavonoids and polyphenols. All these plants are nontoxic at the concentration of 100 mg / ml. The MICs and MBCs of the active extracts varied according to the extracts and bacterial strains studied. The ethanolic extract of Psidium guajava leaves showed bactericidal activity on the strains of Pseudomonas aeruginosa, Salmonella cholereasius and Shigella flexneri. The ethanolic extracts of the leaves of Cajanus cajan and Vernonia amygdalina showed showed bactericidal activity on the strain of Shigella flexneri.
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